The SSR Genetic Diversity of Wild Red Fruit Lycium (Lycium barbarum) in Northwest China

Through a comprehensive regional systematic collection, we conducted genetic diversity analysis of wild red-fruited Lycium resources across the entire northwest region China. This study provides valuable basis for germplasm exploration and selection new varieties. Utilizing fluorescence capillary electrophoresis, carefully screened 16 pairs SSR primers exhibiting high polymorphism. Subsequently, inferred through structure clustering, UPGMA analysis, molecular AMOVA. The 113 barbarum samples collected from China exhibited distinct subgroups, namely Qinghai–Gansu–Ningxia subgroup Xinjiang subgroup. These subgroups were clearly distinguishable based on clustering. within was remarkably rich, as indicated by mean I value 1.04, He 0.57, PIC 0.73. Notably, majority (72.99%) found populations, signifying substantial intrapopulation variation. Furthermore, our findings revealed significant differentiation among with Fst 0.27 gene flow Nm estimated at 0.68. suggests that variation levels in notably high, primarily driven pronounced populations. Nonetheless, it is important to note predominantly persists observed subpopulation regions can be attributed geographical isolation. barriers have played pivotal role shaping populations region. Consequently, sheds light complex landscape Northwest highlights significance considering both within-population population conservation breeding programs.